Human CCL21/6Ckine ELISA KIT


Human CCL21/6Ckine ELISA KIT





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Product name Human CCL21/6Ckine ELISA KIT
Catalog no CHE0140
Detection method Colorimetric
Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 31 pg/mL
Assay range 62.5- 4000 pg/mL
Assay length 3.5 hours
Assay procedure Multiple steps standard assay
Specificity Reacts with: Human
Product information The Human CCL21/6Ckine ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of CCL21/6Ckine in human plasma, serum, and cell culture samples.This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CCL21/6Ckine in approximately 3.5 hours. A polyclonal antibody specific for human CCL21/6Ckine has been pre-coated onto a 96-well microplate with removable strips. CCL21/6Ckine in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CCL21/6Ckine , which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Tested application Suitable for: Sandwich ELISA
Format Pre-coated microplate (12 x 8 well strips)
Storage Store at 2 – 8℃. Please refer to protocols.
Components 1 x 96 tests
Antibody-coated microplate 1 unit
Standard (Lyophilized) 1 x 2 vials
Antibody conjugate concentrate 1 x 2 vials
Streptavidin-HRP solution 1 x 2 vials
Standard/sample diluent 1 bottle
Antibody diluent buffer 1 bottle
Streptavidin-HRP diluent 1 bottle
Wash Buffer Concentrate 20X 1 bottle
Substrate solution 1 vial
Stop solution 1 vial
Adhesive Films 4 pieces

image of Human CCL21/6Ckine ELISA KIT

Representative standard curve for CCL21/6Ckine ELISA. CCL21/6Ckine was diluted in serial two-fold steps in Sample Diluent.

应用 Ab评论 说明
IHC-Fr 1/200.
CHIPseq Use at an assay dependent concentration.
Dot blot 1/10000.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron 1/50. Customer feedback
ChIP Use 2µg for 106 cells
IP Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
ChIP/Chip Use at an assay dependent concentration.
IHC - Use at an assay dependent concentration. PubMed: 22219645
ICC 1/100 - 1/500.

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