Morpholinos属于第三代反义寡核苷酸,由Gene Tools公司独家研制、开发和提供合成服务。
Morpholinos主要通过阻断蛋白质翻译过程而达到抑制目标基因功能的作用,目前已经广泛用于发育生物学和细胞学实验中,并取得了非常好的实验效果。
Morpholinos以吗啉环类似物为基本骨架,这种独特的结构特性使得Morpholinos具有以下几个特点:
n 与其他类型的反义寡核苷酸相比,Morpholinos能更好地抵抗核酸酶的作用
n Morpholinos与靶序列结合后通过空间位阻效应发挥作用,不激活 RNaseH,不引起目标基因mRNA 的降解
n 针对翻译起始位点区设计的Morpholinos用于抑制蛋白质的翻译,针对pre-mRNA剪切位点区设计的Morpholinos可影响mRNA剪切,得到不同的转录本,可以方便地用于目标基因结构域的突变研究
n 与靶序列结合能力强,可以渗入mRNA的二级结构,并且特异性好
配合Morpholinos的使用,Gene Tools公司开发了相应的输送系统,可以安全、高效地将Morpholinos 输送至胞浆内,因此,Morpholinos 也可以用于遗传学研究和新药靶标的发现以及验证。此外,Morpholinos可以标上荧光基团、亲和标签以及活性反应基团,以供不同的研究需要。
特色产品
产品名称 |
规格 |
Custom sequence Morpholino oligo(for any length up to 25 bases.) |
300 nmol |
Custom sequence Morpholino oligo(for any length up to 25 bases.) |
1000 nmol |
Custom sequence Morpholino oligo(for any length up to 25 bases.) |
6000 nmol |
Custom sequence Morpholino oligo(per additional base over 25 bases) |
300 nmol |
Custom sequence Morpholino oligo(per additional base over 25 bases) |
1000 nmol |
Custom sequence Morpholino oligo(per additional base over 25 bases) |
6000 nmol |
Standard oligo modifications |
300 nmol |
Standard oligo modifications |
1000 nmol |
Standard oligo modifications |
6000 nmol |
Custom sequence Vivo-Morpholino |
400 nmol |
Custom sequence Vivo-Morpholino |
2000 nmol |
Custom sequence Vivo-Morpholino (per additional base over 25 bases) |
300 nmol |
Custom sequence Vivo-Morpholino (per additional base over 25 bases) |
1000 nmol |
Custom sequence Vivo-Morpholino (per additional base over 25 bases) |
6000 nmol |
Custom sequence Photo-Morpholino |
300 nmol |
Custom sequence Photo-Morpholino |
1000 nmol |
Endo-Porter (in DMSO) for roughly 150ml culture medium |
ea |
Endo-Porter aqueous for roughly 150ml culture medium |
ea |
Sarstedt cell scrapers |
bundle of 20 scrapers |
Standard control oligo |
100 nmole |
Standard control oligo |
300 nmol |
Std. control with fluorescein |
100 nmole |
Std. control with fluorescein |
300 nmol |
Std. control with lissamine |
100 nmole |
Std. control with lissamine |
300 nmol |
Danio rerio chordin with fluorescein |
100 nmole |
Xenopus laevis beta-catenin with fluorescein |
100 nmole |
Danio rerio p53 |
100 nmole |
Xenopus laevis p53 |
100 nmole |
Random control oligo 25-N |
100 nmole |
GFP |
100 nmole |
gal4-uas |
100 nmole |
Standard control Vivo-Morpholino |
100 nmole |
Standard control Vivo-Morpholino |
300 nmol |
gal4-uas Vivo-Morpholino |
100 nmole |
gal4-uas Vivo-Morpholino |
300 nmol |
GFP antisense Photo-Morpholino |
100 nmole |
gal4-uas antisense Photo-Morpholino |
100 nmole |
gal4-uas sense Photo-Morpholino |
100 nmole |
genetools direct:
bilibili: